Premium
Postnatal jejunal expression patterns of four major house‐keeping genes in pigs are measured by the real time RT‐PCR
Author(s) -
Yang Chengbo,
Li Tiejun,
Yin Yulong,
Fan Ming Z
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1076-c
Subject(s) - hypoxanthine guanine phosphoribosyltransferase , housekeeping gene , biology , real time polymerase chain reaction , reference genes , gene , gene expression , hypoxanthine phosphoribosyltransferase , microbiology and biotechnology , andrology , jejunum , glyceraldehyde 3 phosphate dehydrogenase , genetics , endocrinology , medicine , mutant
The selection of an ideal house‐keeping gene is essential to examine developmental changes in the intestinal gene expression studies by real time RT‐PCR. This study was carried out to examine the postnatal porcine jejunal expression patterns of four major house‐keeping genes, beta‐actin (ACTIN), glyceraldehydes‐3‐phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyl‐transferase (HPRT) and ribosomal protein L10 (RPL10) by real‐time RT‐PCR. Thirty six purebred Yorkshire gilts were used for sampling jejunum at the ages of d 1, 4, 6, 12, 20 and 28 (1 wk post‐weaning). The house‐keeping gene expression, indirectly expressed as threshold cycles (CT) values, was analyzed by real time RT‐PCR (SmartCycler) using the SYBR Green‐I detection kit. Orthogonal polynomial contrasts showed no changes (P>0.05) in ACTIN (16.1–16.8; SE=0.56), GAPDH (19.4–20.6; SE=0.50) and HPRT (28.0–29.8; SE=0.98) expressions but quartic responses in RPL10 (P=0.006; 17.7–18.9, SE=0.25) expression. Our results indicate that beta‐actin, GAPDH and HPRT are the ideal housekeeping genes to examine porcine jejunal gene ontogeny research. Supported by NSERC of Canada and the Chinese Academy of Sciences.