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Porcine jejunal alkaline phosphatase gene expression is quadratically changed during the postnatal growth examined by the quantitative real‐time RT‐PCR
Author(s) -
Li Tiejun,
Yang Chengbo,
Lackeyram Dale,
Yin Yulong,
Fan Ming Z
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1076-b
Subject(s) - alkaline phosphatase , real time polymerase chain reaction , gene expression , biology , housekeeping gene , endocrinology , andrology , weaning , medicine , jejunum , microbiology and biotechnology , gene , enzyme , biochemistry
Intestinal alkaline phosphatase (AP) is responsible for hydrolyzing phosphoric ester bonds of organic compounds and likely plays a role in intestinal transcellular nutrient transport. This study was conducted to examine porcine jejunal AP gene expression (mRNA) during the postnatal growth. A total of 36 littermate purebred Yorkshire gilts were divided into six groups at the ages of d 1, 4, 6, 12, 20 (suckling) and 28 (1 wk post‐weaning), respectively. Relative jejunal AP mRNA abundance was measured by the real time RT‐PCR analysis (SmartCycler) using the SYBR Green‐I detection kit with glyceraldehydes‐3‐phosphate dehydrogenase (GAPDH) as the housekeeping gene. Orthogonal polynomial contrast analyses of the data showed a quadratic pattern (P=0.013) of the porcine jejunal AP mRNA expression (LSMEAN, 1.43, 2.50, 2.90, 4.32, 3.27 and 0.38, respectively, with a pooled SD=2.76) at the ages of d 1, 4, 6, 12 and 20, and 28. These results suggest that porcine jejunal alkaline phosphatase gene expression was increased from birth to the peak at 12‐d suckling and dramatically decreased during the weaning between d 21 and 28. Supported by the Chinese Academy of Sciences and NSERC.