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Transepithelial current and nutrient absorption is altered following an intra‐nasal Salmonella typhimurium challenge and is diet dependent
Author(s) -
Radcliffe John Scott,
Saddoris Kari,
Sims Lindsay,
Aldridge Blaire,
Walsh Maria
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1075
Subject(s) - salmonella , absorption (acoustics) , basal (medicine) , chemistry , weanling , microbiology and biotechnology , medicine , endocrinology , biology , bacteria , genetics , physics , acoustics , insulin
Weanling (19d) pigs (n=88) were used in a 14 d study to evaluate water delivery of direct‐fed microbials (DFM) or organic acids or in‐feed antibiotics on nutrient absorption following an intra‐nasal Salmonella typhimurium challenge. Pigs were allotted to treatments and challenged with salmonella 6 d later. Prior to the salmonella challenge and on d 2, 4, and 8 post‐challenge pigs (5–6/trt) were harvested for collection of jejunal tissue to determine basal short‐circuit current (Isc) and nutrient absorption in modified Ussing chambers. Basal Isc (31.9 to 19.2 μA/cm 2 ) and Na + ‐dependent glucose (56.2 to 29.9 μA/cm 2 ) and P absorption (24.1 to 11.6 μA/cm 2 ) linearly decreased (P < 0.03) from 0–8 d post‐salmonella challenge. A trend (P < 0.06) for a Trt x Day interaction was observed for P absorption. Glutamine absorption increased from d 0 to 2, and then decreased through d 8 post‐salmonella challenge, while Cl − secretion following carbachol treatment remained unchanged (P > 0.10). Glutamine absorption was higher (P < 0.05) for NC pigs compared to DFM pigs, and basal Isc and P absorption tended (P < 0.10) to be higher for NC pigs compared to DFM and antibiotic (P only) fed pigs.