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Effects of Cd on the subcellular localization of transition metals in Saccharomyces cerevisiae
Author(s) -
Zahler Nathan H.,
Stoddard Andrea K.,
Kidd Matthew,
Hernick Marcy,
PennerHahn James,
Fierke Carol A.
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1067-b
Cd is a toxic heavy metal found in industrial contamination and cigarette smoke. While the clinical effects of Cd toxicity are well characterized, their cellular origins remain unclear. One proposed mechanism for cellular Cd toxicity is the displacement of Fe and Zn from metalloproteins. To better understand the impact of Cd on metal homeostasis, we have studied the effect of Cd on the concentrations and localizations of elements including P, S, K, Mn, Fe, Ni, Cu, and Zn in S. cerevisiae using X‐ray nanoprobe imaging. For yeast grown in minimal media in the absence of Cd, P, K, and Mn are concentrated in the vacuole; Fe and Zn are concentrated in both the vacuole and the nucleus; and Cu and S are distributed throughout the cell. Consistent with previous studies, upon exposure to Cd we observe an increase in the overall cellular concentration of Zn and Fe; however, there is no change in the distribution of Fe and Zn. In contrast, we observed a marked redistribution of Cu to the periphery of buds and bud‐necks of dividing yeast when Cd is added to the media. This result demonstrates that Cd impacts Cu metabolism and suggests that displacement of Cu from metalloproteins may also be a factor in cellular Cd toxicity. Supported by NIH grant GM070545 (CAF & JPH) and NIH postdoctoral fellowship ES013881 (NHZ). Advanced Photon Source use was supported by US DOE, Office of Science, Office of Basic Energy Sciences, Contract No. W‐31‐109‐ENG‐38.