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Consumption of a high fat diet increases glucose uptake but not SGLT‐1 expression in the small intestine of a procine model of the metabolic syndrome
Author(s) -
Saddoris Kari L.,
Gabler Nick,
Spurlock Michael,
Radcliffe John Scott
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1054-d
Subject(s) - endocrinology , medicine , jejunum , basal (medicine) , insulin resistance , triglyceride , biology , small intestine , carbohydrate metabolism , glucose uptake , metabolism , insulin , cholesterol , chemistry
Ossabaw pigs are predisposed to the metabolic syndrome, which includes obesity, insulin resistance, and increased blood glucose, cholesterol, and triglyceride concentrations. The objective of this experiment was to determine the effects of feeding a high fat diet to adult Ossabaw pigs on Na + ‐dependent glucose uptake and mRNA expression of SGLT‐1 in the jejunum. Twelve pigs (66.8 ± 3.05 kg) were fed a control diet either ad libitum (n=4) or at the maintenance requirement (n=4), or a high fat diet ad libitum (n = 4). After 20 weeks, jejunal segments were removed and mounted in modified Ussing chambers. Basal resistance (P<0.10) was 27% greater and basal short‐circuit current (P<0.06) was 55% lower in pigs fed the high fat diet compared with pigs fed the control diet either ad libitum or at maintenance. Na + ‐dependent glucose uptake decreased (P<0.02) from 18.47 μA/cm 2 to 3.011 μA/cm 2 as fat consumption was increased. Na + ‐dependent phosphate, amino acid, or dipeptide uptake did not differ (P>0.10) between control and high fat fed pigs. Jejunal SGLT‐1 mRNA expression declined 35 and 62% in pigs fed the high fat diet and limit fed the control diet, respectively, compared to control ad libitum fed pigs, though this difference was not significant (P>0.10). Overall, the feeding of a high fat diet resulted in an 84% reduction in Na + ‐dependent glucose uptake, independent of a change in SGLT‐1 mRNA expression in the jejunum.