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Characterization of Sirtuin Proteins using RasMol‐RP and Three Dimensional Modeling
Author(s) -
O’Mara Deirdre,
Vershon Andrew K.,
Herman Timothy,
Conti Brooke,
Fernando Michael,
Lashley Danielle,
Li Audrey,
Paton Sarah,
White James,
Horlbeck Max
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1036-a
The Pingry School’s SMART Team (Students Modeling a Research Topic) has worked with Andrew Vershon to design and produce accurate three‐dimensional physical models of Human SirT2 using state of the art rapid prototyping technology. SirT2 is NAD‐dependent histone deacetylase that are points of regulation of transcriptional silencing. Sir2 and SirT2 have been characterized to form regulator complexes with other silent information regulator proteins and is analogous by sequence similarity to Hst1 but result in differing function and specificities. Discussion with Dr. Vershon, allowed the Pingry SMART Team to use a modified version of RasMol(RP‐Rasmol)to design physical models of sirtuin proteins highlighting areas of each protein shown to be experimentally significant for function. These final designs were then exported as PLY files and are used to direct an automated ZCorp 3D Color Printer to build physical models of the proteins. These models are communication tools that can be used to enhance the further understanding of these pathways among the scientific community. This is supported by grant awarded to T. Herman by the NIH NCCR SEPA Program.