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MicroRNA expression profiling of adult mouse testis and ovary by microRNAome
Author(s) -
Takizawa Toshihiro,
Mishima Takuya,
Kawahigashi Yutaka,
Luo ShanShun,
Kanda Tomohiro,
Mizuguchi Yoshiaki,
Takizawa Takami
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1028-b
Subject(s) - microrna , biology , computational biology , ovary , real time polymerase chain reaction , messenger rna , gene expression profiling , rna , small rna , in situ hybridization , cloning (programming) , microbiology and biotechnology , gene expression , genetics , gene , computer science , programming language
MicroRNAs (miRNAs) are approximately 22 nucleotide noncoding RNA molecules that play important roles in many biological processes by repressing mRNA translation. MicroRNAome that consists of mass miRNA cloning and sequence analysis, differential expression analysis by real‐time PCR, and in situ hybridization, is a powerful tool for miRNA expression profiling in cells and tissues of interest, and has a significant role to study miRNA functions and to discover new miRNAs in functional genomics. We’ve developed a simple, rapid and efficient microRNAome method based on double PCR amplification of miRNAs. We’ve also improved real‐time PCR methods for the semi‐quantitative analysis of miRNA expression (Brain Res ( in press ), 2007). In this study, we investigated the expression profiles of miRNAs in adult mouse testis and ovary. All experiments were conducted according to the Guide for the Care and Use of Laboratory Animals of Nippon Medical School. We obtained total 22,620 small RNA clones. There were 6,313 miRNA clones (116 kinds) in the testis, 10,118 miRNA clones (120 kinds) in the ovary, and some novel miRNAs. The most expressive miRNA was miR‐125b in both reproductive organs. Real‐time PCR analysis revealed that miR‐125 was highly expressed in the ovary compared to other organs (e.g., lung, heart, liver). The methods for microRNAome reported in this study would be useful as an additional tool for miRNA research.