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MicroRNA Microarray Analysis of Rat Lung‐specific MicroRNAs
Author(s) -
Wang Yang,
Weng Tingting,
Gou Deming,
Chen Zhongming,
Chintagari Narendranath Reddy,
Liu Lin
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1027-e
MicroRNAs (miRNAs) are ~21‐nucleotide small non‐coding RNAs that function in negatively regulating the expression of genes and proteins. The known functions of miRNAs in animals include: timing of developmental transitions, regulation of cell proliferation and differentiation, diseases and cancers, regulation of fat and lipid metabolism, and modulation of insulin secretion. However, to date, the functions of miRNAs in the lung remain largely unknown. In this study, we utilized a rat miRNA microarray containing 216 miRNA probes, printed in‐house, to detect the expression of miRNAs in the rat lung compared to the rat heart, brain, liver, kidney and spleen. Statistical analysis using Significant Analysis of Microarray (SAM) and Tukey Honestly Significant Difference (HSD) Test revealed 2 miRNAs (miR‐195 and miR‐200c) expressed specifically in the lung and 9 miRNAs co‐expressed in the lung and another organ. 12 selected miRNAs were verified by Northern blot analysis. These identified lung‐specific miRNAs will facilitate functional studies of miRNAs during normal physiological and pathophysiological processes of the lung. (Supported by NIH R01 HL‐052146, R01 HL‐071628, and R01 HL‐083188)