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sRNAvalet TM Transfection Reagent
Author(s) -
Prata Carla
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1027-d
Subject(s) - gene silencing , rna interference , transfection , small interfering rna , chemistry , cationic polymerization , microbiology and biotechnology , cell , reagent , rna , gene expression , in vitro , gene delivery , biophysics , macromolecule , gene , biochemistry , biology , organic chemistry
The potential of RNAi as a therapeutic strategy for genetic diseases is becoming more and more evident. RNAi involves the targeted post‐transcriptional degradation of the mRNA thereby inhibiting the synthesis of the desired protein. This leads to silencing of the gene expression. The keys intermediates of this process are short interfering RNA (siRNA) duplexes. However, delivery of these macromolecules into diverse cell types remains a critical challenge. sRNAvalet ™ is a new reagent for the delivery of siRNA that incorporates the latest developments in surfactant science and cellular mechanism. sRNAvalet ™ results in hghly selective gene knowdown in vitro with no toxicity. sRNAvalet ™ is based on a charge‐switchable lipid formulation that transforms from a cationic species into an anionic species. In the cationic state, sRNAvalet ™ initiates an electrostatic complex with siRNA and promotes cellular uptake through the plasma membrane. Once inside the cell, sRNAvalet ™ undergoes a transformation to an anionic state that releases the siRNA and allows efficient gene knowdown.