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Comparison of A‐to‐I RNA editing in wild type strains, Canton S and Oregon R, of Drosophila melanogaster
Author(s) -
Smith Lee Ann,
Pituch Katarzyna,
Moy Hawnyeu,
Nadolski Jeremy
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1026-a
Subject(s) - rna editing , rna , drosophila melanogaster , biology , adenosine , drosophila (subgenus) , inosine , wild type , genetics , biochemistry , mutant , gene
Adenosine to Inosine (A‐to‐I) RNA editing occurs in ion channels, receptors, and synaptic machinery in the nervous system of fruit flies. It has been proposed that RNA editing of these targets in the nervous system can affect fast transmission and help fine tune responses. We have utilized the wild type strain, Canton S (CS), for studies involving changes in RNA editing levels after ethanol (EtOH) exposure. These flies exhibit the typical intoxicated behavior after 40 minutes of exposure to 70% EtOH: brief period of hyperactivity followed by sedation. RNA from the flies is extracted at various time points after a single exposure and subjected to RT‐PCR followed by sequencing of the products. Six hours after EtOH exposure, levels in RNA editing are altered, most notably in the editing enzyme, dADAR ( D rosophila A denosine D e a minases acting on RNA). Using the wild type strain, Oregon R (OR), in the same EtOH exposure paradigm, the flies displayed a greater tolerance for EtOH as reported by others. We are investigating the levels of RNA editing of both strains to determine if the differences exist between CS and OR and if both strains respond similarly with exposure to EtOH.