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Genetic Interaction mapping of essential genes in Saccharomyces cerevisiae
Author(s) -
Huang Jiahao,
Krogan Nevan,
Schuldiner Maya
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1004-c
Subject(s) - epistasis , gene , genetics , biology , saccharomyces cerevisiae , phenotype , mutation , candidate gene , gene mapping , chromosome , computational biology
Genetic interactions measure how mutation in one gene contributes to the phenotype afflicted with a second gene mutation. Previous genetic mapping of Saccharomyces cerevisiae using E‐MAPs (epistatic miniarray profile) have revealed great insight into the functions of previously characterized and uncharacterized non‐essential genes. However, systematic genetic analysis of essential genes has been hard to achieve because deletion of essential genes leads to cell death. We have developed a new technique, termed DAmP (Decreased Abundance by mRNA Perturbation), where hypomorphs of essential genes are created by disruption of their 3′UTR sequences. We have generated a quantitative genetic map of 96 essential genes involved in various aspect of chromosome functioning and regulation using DAmP and E‐MAP technology to reveal genetic interactions within this essential biological process.