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Analysis of cytLEK1 interaction with hook2
Author(s) -
Moynihan Katherine,
Pooley Ryan,
Soukoulis Victor,
Bader David
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a80-b
Subject(s) - centrosome , microtubule organizing center , colocalization , microbiology and biotechnology , microtubule , motility , cell division , centrosome cycle , biology , function (biology) , cell , chemistry , cell cycle , genetics
The centrosome is the microtubule organizing center (MTOC) of the cell and plays a primary role in cell division and motility. The linker protein hook2 has been shown to localize to the centrosome and function in microtubule (MT) organization and growth. Potential binding partner cytLEK1 has been shown to interact with the MT network and colocalize with hook2 in COS‐7 cells. The interaction between these two proteins is a possible physical link from the MT network to the centrosome. Thus far, yeast‐two‐hybrid screens, biochemical analysis, and immunofluorescence colocalization studies provide evidence indicative of this physical interaction. Studies are underway to determine the significance of disrupting this interaction on cell structure and motility. Utilizing newly developed cytLEK1 KO fibroblast cells to investigate possible regulation of cell movement and division will be employed.