Premium
Similarity of bioactivity between purified and semipurified glucoraphanin
Author(s) -
Lai RenHau,
Jeffery Elizabeth H.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a733-c
Subject(s) - glucoraphanin , cecum , cruciferous vegetables , sulforaphane , chemistry , myrosinase , kidney , food science , pharmacology , glucosinolate , biochemistry , medicine , endocrinology , biology , botany , brassica , cancer
The anti‐carcinogenic effects of cruciferous vegetables have been attributed to the hydrolysis products of glucosinolates; the primary glucosinolate in broccoli is glucoraphanin (GP). In this study, the goal was to determine if purified GP, in the absence of the plant‐derived hydrolyzing enzyme myrosinase, could affect pulmonary or hepatic ethoxyresorufin O‐deethylase (EROD) and/or quinone reductase (QR) activity. Gavage of male F344 rats with semi‐purified or purified GP (240 mg/kg rat daily for 4 days) caused similar changes in QR and no change in EROD. In a second study, varying doses of semi‐purified GP (0, 30, 60, or 120 mg/kg rat daily for 4 days) caused no change in EROD activity, but a dose‐dependent increase in QR. In addition, the GI tract, liver, lung, kidney and bladder all exhibited normal histopathology, except cecum. The cecum from rats receiving 120 mg/kg daily for 4 days showed some inflammation and those receiving 240 mg/kg showed extensive inflammation. Urine analysis by HPLC/UV was greater on day 4 than day 1 of administration, and the lower the dose, the greater the recovery. We conclude that GP 30 and 60 mg/kg, daily for 4 days, are safe and effectively enhance QR in all tissues evaluated. This research was funded by Kraft Foods Ltd