Premium
Amelioration of Acute Pancreatitis with Dietary Crambene from Crucifers
Author(s) -
ArayaRoldan Marcela M.,
Jeffery Elizabeth H.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a728-b
Subject(s) - acute pancreatitis , pancreatitis , cholecystokinin , medicine , amylase , lipase , endocrinology , pancreatic injury , in vivo , pancreas , glutathione , chemistry , biology , enzyme , biochemistry , receptor , microbiology and biotechnology
Acute pancreatitis is a common and refractory inflammatory disease affecting pancreatic acinar cells. The mortality rate is approximately 30%. Cholecystokinin (CCK) ‐induced acute pancreatitis is the most widely used in vivo experimental model. Administration of crambene (1‐cyano‐2S‐hydroxy‐3‐butene) to male F344 rats induces glutathione synthesis and apoptosis in pancreas. Furthermore, studies conducted in mice suggest that crambene may reduce the severity of experimental acute pancreatitis. Therefore, we hypothesized that dietary pretreatment of male F344 rats with crambene will ameliorate acute pancreatitis by inhibiting inflammatory mediators. CCK (100 mg/kg s‐c × 2, one h apart) caused significantly elevated serum amylase (3‐fold) and lipase (8‐fold) 2 h after the last injection, confirming pancreatitis. Administration of crambene (300 and 600 μmol/kg) 12 h prior to CCK, significantly reversed the elevation in the serum amylase and lipase activities to 1.5‐fold and 1.1‐fold, respectively (p< 0.05). Crambene similarly decreased the CCK‐induced elevation in the pancreatic pro‐inflammatory cytokines IL‐6 and TNF‐α. We conclude that crambene from dietary crucifers may be useful for prevention of acute pancreatitis. Supported by USDA, NRI 05‐02622