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Phytochemical from Centella asiatica attenuated MAPKinase and COX‐2 in Caco‐2 human intestinal cells induced inflammation by lipopolysaccharide (LPS
Author(s) -
Muangnoi Channarong,
Siriprapa Pussadee,
Kriengsinyos Wantanee,
Chitchumroonchokchai Chureeporn
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a726-c
Subject(s) - centella , lipopolysaccharide , traditional medicine , caco 2 , chemistry , inflammation , western blot , phytochemical , pharmacology , biology , biochemistry , medicine , cell , immunology , gene
Centella asiatica is widely used as traditional medicine in Asia. Centella asiatica like other vegetables and herbs that contain various kinds of phytochemicals especially triterpenoids including asiaticoside, madecassoside, madecassic acid, and asiatic acid. These bioactive compounds are also quoted in the protective effect of anti‐inflammatory. Thus, this study examined anti‐inflammatory of phytochemicals from crude extracted of Thai fresh Centella asiatica . Caco‐2 human intestinal cells were pre‐treated with crude hot water or organic extract of Centella asiatica for 4h. Morphology of monolayer was not change under microscopic observation and also protein content was not different between pre‐treatment and control. The change of MAPKinase; phospho‐JNK and phospho‐p38 and COX‐2 proteins in these cells with the activation of 1μg lipopolysaccharide (LPS)/ mL test medium for 30 min were measured. The expression of JNK, p38 and COX‐2 were determined using electrophoresis and subjected to western blot. The results showed that LPS induced inflammation by stimulated COX‐2 and MAPKinase resulting in phosphorylation of JNK and p38 proteins. Water and organic solvent extract of Centella asiatica were not different in inhibition phospho‐JNK. However, organic solvent extraction was more potent to reduced phospho‐p38 and COX‐2 (50 and 47% reduction, respectively) than water extract (18 and 38% of reduction) of fresh Centella asiatica . These data demonstrated that crude extracted of Thai fresh Centella asiatica effectively attenuated LPS‐induced inflammation in Caco‐2 human intestinal cells using MAPKinase and COX‐2 as marker. (Supported by Graduate Program, Mahidol University and National Research Council of Thailand)