Premium
Decreasing postnatal skeletal muscle protein synthetic activity is associated with a reduction in the relative expression of phosphorylated eEF2 in fed pigs
Author(s) -
Yang Xiaojian,
Fan Ming Z
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a714-d
Subject(s) - skeletal muscle , western blot , phosphorylation , medicine , saline , endocrinology , chemistry , zoology , biology , andrology , biochemistry , gene
This study was conducted to investigate the association between developmental changes of fractional protein synthesis rates (FSR) and the expression of eukaryotic protein synthesis elongation factor 2 (eEF2) in the skeletal muscle of fed pigs. Thirty‐six purebred Yorkshire gilts were used at the ages of d 1, 4, 6, 12 and 20 (suckling) as well as d 28 (1 wk post‐weaning). Piglets were given intraperitoneally a flooding dose of Phe containing L‐[ring‐ 2 H 5 ]Phe in saline. Plasma and loin muscle samples at 30 min post‐injection were collected for the determination of tracer Phe enrichment by GC‐MS. Total and phosphorylated (Thr56) forms of eEF2 were examined by Western blot. The FSR of skeletal muscle decreased linearly (P<0.05) from d 1 (20.8 %/d) to day 28 (5.3 %/d). Both total and the phosphorylated forms of eEF2 abundances were linearly decreased (P<0.05) and were correlated with FSR (P<0.05; r= 0.48 and 0.53) at the ages of d 1 to 28. However, the ratio of the phosphorylated eEF to total eEF abundance was linearly increased (P<0.05). These results indicate that the decreasing FSR of skeletal muscle are associated with a reduced relative expression of the phosphorylated eEF2 in fed pigs during the postnatal growth. Supported by NSERC of Canada.