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Combined Stable Isotope and Positron Emission Tomography for in vivo Metablic Investigation‐ Validation in an Animal Model
Author(s) -
Yu YongMing,
Bischoff David,
Cabral Carolina,
Lin Florence Min,
Carter Edward A,
Kelleher Joanne K,
Tompkins Ronald G.,
Fischman Alan J
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a699
Subject(s) - positron emission tomography , in vivo , supine position , nuclear medicine , isotope , metabolism , blood flow , tomography , chemistry , pet imaging , carbohydrate metabolism , preclinical imaging , medicine , biology , biochemistry , radiology , physics , microbiology and biotechnology , quantum mechanics
Positron Emission Tomography (PET) is an image technique based on acquisition of tissue images by detecting radiation from the emitted positrons. It differentiats the metabolism between normal and tumor tissues. We combined PET with stable isotope (SI) to quantify substrate metabolism in specific tissues and whole body (WB)in vivo. Seven rabbits were studied. They were surgically prepared and placed supine under the gantry of PET camera. The tomographic slice was positioned to include both hind limbs (HLs). C15O2 was inhaled for imaging blood flow (BF) and 18FDG was injected followed by serial images for muscle glucose metabolism (GM). [6,6, 2H2]‐glucose was infused simultaneously for GM in WB and HL by A‐V balance. The resutls (mean± SE): WB GM: 2.54±0.22 mg/kg/min, GM in HL: 0.77 ± 0.12 mg/leg/min. the measured muscle GM: 3.40±0.46 ìg/g muscle/min by SI and 3.64±0.22 by PET (NS t‐test). BF by C15O2 was 0.059±0.010 ml/g/min. Conclusion: PET‐SI is a powerful tool for non‐invasive in vivo metabolism investigtions.