Premium
L‐lactate decreases calcium uptake in SH‐SY5Y cells with defective ATP production
Author(s) -
Bataineh Mo'ath,
Yamada Naomi,
Khosla Pramod
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a691-b
Subject(s) - calcium , incubation , oligomycin , endocrinology , medicine , chemistry , calcium metabolism , biochemistry , biology , enzyme , atpase
Alzheimer's disease (AD) is a major source of morbidity in the elderly, and exercise is thought to delay its onset. Since AD is associated with defective ATP production and higher than normal cytoplasmic calcium levels, we initially sought to determine whether lactate (a product of exercise) is associated with reduced calcium uptake. Calcium uptake measured in pg/mg protein, was determined by incubating human neuroblastoma cells (SH‐SY5Y) with 45‐Ca in the absence, or presence of 4 μg Oligomycin, and various concentrations of lactate (0 to 20 mM). Cells were incubated for 1, 4, 8, 12, 24 or 48 hrs. Calcium uptake followed a general trend that showed a peak uptake with 2 mM lactate followed by declines at 10 and 20 mM lactate. A lactate concentration of 2 mM significantly stimulated calcium uptake from baseline at 4, 8, 24, and 48 hrs. Higher lactate concentrations significantly reduced calcium uptake at 1, 4, 8, and 24 hrs. Oligomycin significantly increased calcium uptake following a 1, 4, 12, and 48 hr incubation; but reduced uptake following 8 hour incubation. These data suggest that: higher lactate concentrations are associated with lower calcium uptake, which is consistent with the positive effects of exercise seen in AD.