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Development of a Panel of Microsatellite Markers for the Assessment of Genetic Structure in White‐Tailed Deer in Northern Illinois and Southern Wisconsin
Author(s) -
Kelly Amy Catherine,
MateusPinilla Nohra,
Beissel Tom,
Diffendorfer Jay,
Killefer John,
Novakofski Jan,
Shelton Paul
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a67
Subject(s) - chronic wasting disease , microsatellite , biology , genetic structure , population , genetic marker , allele , genetic variation , genetics , disease , medicine , gene , environmental health , prion protein , pathology , scrapie
Microsatellite markers are an effective tool to assess genetic relationships between individuals in a population and evaluate gene flow. Genetic information can be obtained from microsatellite markers using fewer samples than with most other genetic assessment techniques. The objective of this study was to use a panel of microsatellite markers to examine genetic profiles of white‐tailed deer in a region of Northern Illinois and Southern Wisconsin. Chronic Wasting Disease (CWD), a cervid transmissible spongiform encephalopathy, was identified in this area in 2002 and examining genetic structure may help determine familial and environmental components of CWD transmission. Thirty‐two microsatellite markers were examined, which had been previously shown to have multiple alleles in deer (3 to 22). Optimized and multiplexed PCRs were completed on male and female deer from within and adjacent to CWD positive areas. Deer were sampled as part of ongoing CWD surveillance. Landscapes sampled included urban, forest and crop land. We hope to utilize genetic profiles to predict deer movement and relatedness, factors that could aid in the prediction of CWD spread across the landscape. Research funded by USGS and IDNR.