Premium
DIFFERENT SUSCEPTIBILITY OF MITOCHONDRIAL RESPIRATORY CHAIN FOR NITRIC OXIDE IN DIFFERENT ORGANS DURING HYPERTENSION
Author(s) -
CalderónCortés Elizabeth,
CortésRojo Christian,
ClementeGuerrero Mónica,
GutiérrezPérez Areli,
EsquivelGutiérrez Edgar,
CaudilloNoriega Evangelina,
NoriegaCisneros Ruth,
ManzoÁvalos Salvador,
SaavedraMolina Alfredo
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a668-b
Subject(s) - mitochondrion , cytochrome c oxidase , cyanide , kidney , nitric oxide , coenzyme q – cytochrome c reductase , cytochrome , respiratory chain , chemistry , cytochrome c , endocrinology , medicine , oxidase test , respiratory system , biochemistry , pharmacology , biology , enzyme , inorganic chemistry
Mitochondria are an energetic source of cells by means of the formation of ATP synthesis. ATP synthesis is regulated by different mechanisms and molecules as nitric oxide (NO). NO and its derivatives inhibit mitochondrial respiration in a reversibly fashion in the binuclear O2 binding site of the cytochrome c oxidase. In aging and hypertension NO is associated with mitochondrial dysfunction. We performed experiments to investigate if the activity of cytochrome c oxidase is affected in hypertension in the presence of NO in mitochondria of three organs (brain, liver, and kidney) in spontaneously hypertensive rats of 1 and 7 months old. The activity of complex IV was measure by means of oxygen consumption with an electrode type Clark using inhibitors of complexes I and III. Ascorbate was added as substrate of complex IV and TMPD was used as artificial electron donor. The respiratory rate was decreased in presence of 50 μM SNAP in brain mitochondria, liver and kidney of SHR rats of 7 month old, but not in 1 month old SHR rats. The susceptible inhibition was higher in liver than in kidney. The susceptibility of complex IV to SNAP and cyanide is greater in brain>liver>kidney mitochondria of 7 months old compared to mitochondria of 1 month old rats. These data suggest that susceptibility of cytochrome c oxidase to inhibitors is different between mitochondria of different tissue and hypertension contribute to this sensibility. Acknowledgements: The authors appreciate the partial economic support from the grants of CONACYT (43705) and CIC‐UMSNH (2.16‐2006).