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Translational regulation of specific mRNAs by reproductive hormone GnRH
Author(s) -
Do MinhHa,
Jain Sonia,
Nguyen Kathryn A.,
Santos Sharon J.,
He Feng,
Lawson Mark A.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a650-a
Subject(s) - gonadotropic cell , polysome , translational regulation , gonadotropin releasing hormone , messenger rna , biology , translation (biology) , rna , ribonucleoprotein , gene expression , microbiology and biotechnology , luteinizing hormone , gene , endocrinology , hormone , genetics , ribosome
The neuropeptide gonadotropin‐releasing hormone (GnRH) regulates the synthesis of reproductive hormone luteinizing hormone (LH) in pituitary gonadotropes through regulation of both transcription and translation. This study investigated the ability of GnRH to regulate translation of specific mRNAs. GnRH‐treated LβT2 gonadotrope cell extracts were fractionated to separate mRNAs in actively translating polysomes from mRNAs in translationally inefficient ribonucleoprotein complexes (RNPs). Overall, GnRH stimulation redistributed RNA to RNPs, independent of transcriptional events. Quantitative real‐time PCR and Affymetrix GeneChip were used to examine specific mRNAs. Despite the overall shift of RNA to RNPs, individual transcripts were redistributed differently. Lhb and Cga, subunits of LH, redistributed to RNPs, whereas C‐myc and Egr1 redistributed to polysomes, and the receptor for GnRH was not regulated. Microarray analysis confirmed the regulation of Lhb and identified other regulated genes. The results show that GnRH exerts control at the translational level and differentially targets specific mRNAs, directing their movement in translational complexes. The targeted mRNAs include those important for gonadotrope function, indicating that GnRH utilizes translational control as a regulatory mechanism to modulate physiologically relevant gene expression.