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The Ribonucleotide Reductase system from Bacillus cereus
Author(s) -
Roehr Aasmund Kjendseth,
Sørlie Morten,
Andersson K. Kristoffer
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a640-b
Subject(s) - ribonucleotide reductase , deoxyribonucleotides , ribonucleotide , biochemistry , enzyme , electron paramagnetic resonance , bacillus cereus , chemistry , electron paramagnetic resonance spectroscopy , reductase , biology , nucleotide , protein subunit , bacteria , gene , genetics , physics , nuclear magnetic resonance
The enzyme ribonucleotide reductase (RNR) catalyzes the reduction of the four ribonucleotides to their corresponding deoxyribonucleotides. We have cloned, purified, the two subunits R1 and R2 of Bacillus cereus RNR and characterized the R2 protein by electron paramagnetic resonance (EPR) spectroscopy. Enzyme kinetics and binding studies for the Ribonucleotide Reductase system will also be presented.