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Human securin: a p53 mediated protooncogene
Author(s) -
Wu Chang You,
Chao JuiI,
Chu HsuehLiang,
Chang PeiHsin,
Lo ChiuAn,
Chao HsiuMing,
Chang ChiaChing
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a634-d
Subject(s) - securin , separase , chemistry , isothermal titration calorimetry , anaphase , recombinant dna , microbiology and biotechnology , function (biology) , cell cycle , biology , cell , biochemistry , gene
Human securin, has been recognized as a protooncogene in many different cancers. Meanwhile, it plays a role as a separase inhibitor in cell cycle. However, its structure still remains unknown. In order to realize the structure‐function relationship of human securin in oncogenesis. We have recombinant expressed human securin from E. coli , and refolded it into native state. Circular dichroism spectra analysis indicated that securin might not contain unique secondary structure. However, both far‐western and isothermal titration calorimeter analysis indicated that securin interacted with p53 in vitro . This interaction may indicate that the function of p53 has been blocked with securin and caused tumor genesis.