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DNA microarray analysis of Runx1 and Runx2 overexpressing cells.
Author(s) -
Strom David K.,
Fillppelli Crystal,
Taylor Holland
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a632-d
Subject(s) - transactivation , runx1 , cell cycle , biology , runx2 , gene , gene expression , microarray analysis techniques , haematopoiesis , microbiology and biotechnology , transcription factor , genetics , stem cell
The RUNX family of transcriptional regulators are involved in development, hematopoiesis, bone formation, and cancer. We have shown that over‐expression of Runx1leads to a shortening the G1 phase of the cell cycle. This cell cycle phenotype required both an intact DNA binding domain and transactivation domain. This strongly suggests that Runx1 affects cell cycle progression by altering gene expression. To investigate this hypothesis, we expressed Runx1 and Runx2 proteins in NIH3T3 cells using a retroviral expression system. RNA was isolated at 40 hours post‐infection and DNA microarray analysis was performed. The results of these experiments show a number of genes both up and down regulated by Runx1 and Runx2 over expression. Changes in expression of selected genes have been verified by Northern analysis. Additional experiments are underway to repeat these experiments in a hematopoietic cell line.