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Sensitivity and specificity of optimized immunodetection reagent kits for detection of proteins in reverse‐phase low density microarrays
Author(s) -
Mbwana Mwanatumu,
Panagoulis Daniel,
Sreeram Renjina,
LinskeO'connell Lisa,
Webster Helen,
Levin Joshua
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a632-a
Subject(s) - nitrocellulose , protein microarray , reagent , protein array analysis , chromatography , protein detection , chemistry , membrane , microbiology and biotechnology , chemiluminescence , antigen , biochemistry , biology , dna microarray , nanotechnology , materials science , gene expression , gene , immunology
Protein arrays, especially reverse protein arrays, are powerful tools for parallel analysis of proteins and patient samples. Thin nitrocellulose membranes are widely used as surfaces for reverse arrays because nitrocellulose membranes are convenient and inexpensive, and can be used routinely for immunodetection of protein antigens in an array format. KPL now offers optimized reagent sets for the immunodetection of arrayed proteins. These kits are provided with either colorimetric or chemiluminescent detection and can be used with mouse or rabbit primary antibodies. In this poster, we describe the sensitivity and specificity of these detection kits. The kits enable detection of as little as 30 pg of protein in either detection format with no significant detection of nonspecific proteins. Complex samples such as serum or cell extracts can be used. The kits can also be used in conjunction with ProteoSOL, a reagent that extracts proteins from formalin‐fixed paraffin embedded tissues, to extract and quantitate protein biomarkers from these archival clinical tissue samples.