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Global gene expression profiling of key adipogenic tissues in hypergravity‐exposed lactating rats
Author(s) -
Dover Heather,
Patel Osman V.,
Ronca April,
Wade Charles E.,
Plaut Karen
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a632
Subject(s) - hypergravity , adipogenesis , gene expression profiling , profiling (computer programming) , gene expression , microbiology and biotechnology , chemistry , biology , medicine , gene , genetics , computer science , operating system
Exposure to hypergravity (2g, HG) alters metabolism in lactating rats. Genome‐wide expression analysis was conducted in multiple tissues to identify important pathways affected by HG during the periparturient period. Pregnant rats were exposed to HG from gestation day 11 (G11) through postpartum day 1 (P1) and compared to controls kept at 1g (SC). RNA from mammary gland (MG), liver (LV) and adipose (AD) tissue was collected at P1, amplified (Nugen) and hybridized to Affymetrix rat chips (230 2.0). Data from 30 microarrays revealed a total of 707 (MG), 65 (LV) and 36 (AD) genes were up‐regulated ( p <0.05, fold>1.8); while 589 (MG), 125 (LV) and 68 (AD) genes were down‐regulated ( p <0.05, fold<0.5) in HG compared to SC. Functional annotation categories included cell communication, immune response, transcription factors and macromolecule metabolism. Gene enrichment analysis identified unique sets of common genes; one set encoded for specific enzymes responsible for regulation of lipid metabolism and was altered due to HG. Our data indicates exposure to HG down‐regulates the coordinated expression of key genes associated with the lipogenic pathway during lactation. (Supported by NASA NNA05CP91A)