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Elucidating antibacterial drug action by proteomics approach
Author(s) -
Hwa Kuoyuan,
Chiang DiShuan,
Yao ChenWen
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a625-b
Subject(s) - dna gyrase , antibiotics , drug , mechanism of action , ciprofloxacin , proteomics , drug discovery , antibacterial activity , drug action , computational biology , bacteria , biology , microbiology and biotechnology , bioinformatics , pharmacology , biochemistry , genetics , gene , escherichia coli , in vitro
Proteomic technology has been used to study the physiological response of bacterial cells under different environmental stress. We have used the technology to study the protein profiles of bacterial cells under seven different known antibiotics. We have collected the digital images of two‐dimensional gels from the protein samples of cells treated with the antibiotics. A reference image database is constructed. Our image analyses show that antibiotics with similar mechanism of action have similar image patterns. However, different classes of antibiotics have distinct image patterns. In addition, we have identified 50 spots as markers to classify these antibiotics based on their mechanisms of drug action. Furthermore, we have used our reference database to study a new antibacterial drug 755a, as a lead candidate in our work. We showed that 755a has a similar mechanism with ciprofloxacin which inhibits DNA gyrase. These results suggest that proteomic technology can be used to study drug action of a compound. And, the proteomic technology should provide a useful approach for antibacterial drug discovery.