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Involvement of nitric oxide‐cGMP signaling in Leyding cell stress response
Author(s) -
Andric Silvana A,
Stojkov Natasa J,
Janjic Marija M,
Stojilkovic Stanko S,
Kostic Tatjana S
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a622-c
Subject(s) - soluble guanylyl cyclase , phosphodiesterase , nitric oxide , second messenger system , leydig cell , intracellular , signal transduction , cgmp dependent protein kinase , endocrinology , medicine , nitric oxide synthase , protein kinase a , cgmp specific phosphodiesterase type 5 , messenger rna , cyclic nucleotide , in vivo , chemistry , kinase , microbiology and biotechnology , biology , sildenafil , enzyme , biochemistry , nucleotide , guanylate cyclase , hormone , cyclin dependent kinase 2 , luteinizing hormone , gene
The ability of stress to interfere with the Leydig cell steroidogenesis has been shown previously, but the intracellular signaling pathways involved in this process have not been identified. In this study, we examined the effects of immobilization stress (1x120 min, 2x120 min within 2 days, and 10x120 min within 10 days) on nitric oxide (NO)‐cGMP signaling pathway in rat Leydig cells from adult animals. Purified Leydig cells were obtained form freely moving (controls) and immobilized rats, a procedure that required about 8 hr, and were then subjected to RT‐PCR and functional analyses. Immobilization stress was accompanied with a decrease in mRNA transcripts for phosphodiesterase 5 and multidrug resistance protein 5 in all three experimental groups, and with an increase in transcripts for inducible NO synthase in 1×120‐ and 2×120‐min treatments, whereas the mRNA levels for endothelial NO synthase, soluble guanylyl cyclase, protein kinase G, and cyclic nucleotide‐gated channels were not affected by single and repetitive immobilization. In vivo androgen levels and in vitro Leydig cell steroidogenesis were also affected by 1×120‐ and 2×120‐min treatments, but recovered after 10×120‐min treatment. Experiments with inhibitors of soluble guanylyl cyclase revealed the dual effect of NO on steroidogenesis, inhibitory mediated directly by this messenger, and stimulatory, mediated through cGMP signaling pathway.