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Development of a selection strategy to identify novel genes involved in the S. pombe SREBP pathway.
Author(s) -
Stewart Emerson VanLeuven,
Espenshade Peter J
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a608-c
Subject(s) - sterol regulatory element binding protein , transcription factor , gene , biology , sterol , genetics , microbiology and biotechnology , computational biology , biochemistry , cholesterol
Sterol regulatory element binding protein (SREBP) is a membrane bound transcription factor that regulates sterol homeostasis in mammalian cells. The fission yeast ortholog, Sre1, controls adaptation to low oxygen in response to inhibition of oxygen‐dependent sterol synthesis. Dissection of promoter elements in Sre1 target genes identified the DNA consensus sequence bound by Sre1 which resembles that bound by SREBP in the human LDL receptor promoter. Using this information we developed a genetic selection strategy to identify positive and negative regulators of the sre1 + pathway. A clear understanding of this orthologous pathway will serve to direct our study of the SREBP pathway in mammalian cells and further our understanding of its regulation. Preliminary results have identified genes that are known regulators of the SREBP pathway, thus validating the approach. Research was funded by a grant from the NIH HL‐077588 and a Burroughs Wellcome Fund Career Award in the Biomedical Sciences (PJE).