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Phospholipase D1‐induced phosphatidic acid at the plasma membrane promotes a late stage of large dense‐core
Author(s) -
Vitale Nicolas,
ZeniouMeyer Maria,
ChasserotGolaz Sylvette,
Bailly Yannick,
Bader MarieFrance
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a604-a
Subject(s) - phosphatidic acid , exocytosis , phospholipase d , membrane , chemistry , membrane curvature , microbiology and biotechnology , biophysics , phospholipase , biochemistry , biology , lipid bilayer , signal transduction , phospholipid , enzyme
PA is a bioactive lipid with multiple assigned functions. One of the most intriguing ones is the ability of this lipid to change membrane topology by inducing negative curvature of membranes. Our previous results suggested but not proved a role of PLD1 produced PA in regulated exocytosis from neuroendocrine cells. Using PC12 and chromaffin cells and the siRNA technology, we now show that that endogenous PLD1 is indeed a key component of the exocytotic process. Upon stimulation, PLD1 produces phosphatidic acid at the plasma membrane. Importantly, we were able to show that this lipid is produced near or at exocytotic sites and that its presence is absolutely required for proper exocytosis to occur. Membrane capacitance measurements indicate that PLD1‐produced PA is required for the formation of fusion‐competent granules in chromaffin cells. Finally, we provide evidence that membrane bending induced by local accumulation of PA at the plasma membrane is the mechanism underlying the requirement for PA synthesis during the exocytotic process.