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Serum appearance and disappearance of 3, 4‐didehydroretinol in Sprague Dawley rats fed a normal‐ versus high‐fat diet
Author(s) -
Escaron Anne Laure,
Green Michael H.,
Tanumihardjo Sherry A.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a60
Subject(s) - medicine , endocrinology , obesity , vitamin , chemistry , zoology , biology
The obesity epidemic is now a global problem. The effect of high‐fat diets and adiposity on measures of vitamin A (VA) status has not been determined. To examine these effects, rats were fed two levels of fat and then dosed orally with 3, 4‐didehydroretinyl acetate (DRA). Two indicators were used to assess VA status: total liver retinol (R) and the modified relative dose response (MRDR) test. In vivo , DRA is metabolized to 3, 4‐didehydroretinol (DR). Rats (21 d; n = 48) were randomized to one of 4 treatment groups in a 2 x 2 design and fed high‐fat (21.5%) or normal‐fat (5.6%) diets ad libitum for 77 d. Seven d after arrival, each dietary group was dosed daily with either 0.017 or 0.07 μmol retinyl acetate. From 7 through 13 wk of age, rats fed the high‐fat diet weighed significantly more compared with the rats fed the normal‐fat diet. On d 70, all rats were dosed with DRA (0.7 μmol). Blood (1 mL) was sampled at baseline, 2, 4, 6, 8, 16, 30, 48, 96, 120, and 168 h ( n = 4/treatment group) after the DRA dose. DR was used as a tracer to monitor VA metabolism by treatment group. Livers and fat pads were collected at 96, 120, and 168 h following the dose. Liver and serum samples were analyzed by HPLC for DR and R. High‐fat feeding appears to decrease hepatic concentrations of R, DR, and DR esters (Main effect of fat P < 0.05; in nmol/g), but does not alter VA status assessment as determined by the MRDR test. ILSI Future Leader's Award 2004–2006 and NIHNIDDK RO1 61973.