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Cloning, distribution and differential expression of a crustacean CNS Tyr/Oct Receptor
Author(s) -
VazquezAcevedo Nietzell,
ReyesColon Dalynes,
Rivera Nilsa M,
Ruiz Eduardo A,
Kohn Andrea B,
Moroz Leonid,
Sosa Maria A
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a598-b
Subject(s) - prawn , biology , neuropil , dominance hierarchy , receptor , crayfish , crustacean , agonistic behaviour , zoology , dominance (genetics) , medicine , endocrinology , ecology , central nervous system , gene , aggression , genetics , psychiatry
The freshwater prawn M. rosenbergii is a tropical crustacean with characteristics similar to the lobster and crayfish. Adult males develop through three morphological types (small [SC], yellow [YC] and blue claws [BC]). Each morphotype represents a level in the dominance hierarchy of a group, BC males being the most dominant. We are interested in understanding the role played by biogenic amines in the mechanisms underlying aggressive behavior and the establishment of dominance hierarchies in this type of prawn. We have cloned and sequenced a prawn Tyr/Oct receptor (Tyr mac1 R) and determined its distribution in the CNS. This receptor is 458 amino acids long and 64% homologous to the OctR of the tobacco budworm. Tyr mac1 R immunoreactivity was observed as extensive punctate staining within the neuropil and on the membrane of groups of neurons in all ganglia throughout the CNS. Results from real‐time PCR experiments showed there are significant differences in expression of the Tyr mac1 R in thoracic ganglia, with a similar trend being observed in the brain of the SC males as compared with the BC and YC males. These results may be indicative of a relationship between receptor expression and the mechanism underlying the regulation of submissive behavior and the determination of social status. Supported by: NIH/MBRS S06GM008224, NIH/MRISP MH48190, NIH/RCMI G12RR03051, NIH R01 NS39103