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Significant differences in novel and adapted circadian movement behavior in three parental and two ENU knockout inbred strains of rats
Author(s) -
Feroah Thom R,
Forster H V,
Merritt A,
Dwinell M,
MorenoQuinn C,
Greene A,
Jacob H,
Kwitek A,
Cowley A
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a595-a
Subject(s) - circadian rhythm , morning , inbred strain , biology , medicine , physiology , endocrinology , genetics , gene
Five inbred strains of rats of both genders (BN, FHH, SS, FHH‐Agtr1b m1Mcwi , FHH‐Nr4a1 m1Mcwi ) were screen via a new high throughput system for novel and circadian movement behavior phenotypes. The screening system consisted of 12 plethysmographic chambers with movement behaviors acquired by horizontal and vertical sensors while the rats were exposed to a 12 hrs light:dark cycle (0800:2000 hrs). The screening protocol began at 1200 hrs on the 1 st day and continued until the morning of the 4 th day and was divided into Novel (1 st four 30 min periods of the study) and Adapted (starting with Day 1 dark period) periods and analyzed in 12 hr intervals for analysis. During the 1 st 30 min Novel interval, females of all strains had a significantly greater ((P < 0.05) exploratory behavior (AMEXP) than males of the same strain with SS females greater than other females. Significant differences were seen in thigmotaxis (THG) measurements ENU strains compared to same gender parental controls. Circadian AMEXP values in BN were 2‐3 less than other strains while all strains showed a significant circadian variation. The ENU strains showed significant differences in AMEXP and THG compared to parental controls. While these data shows clear but unexpected differences in behaviors in the studied ENU knockout rats, this information provided the basis for further genetic dissection of these behaviors with more directed behavior measurements. This study was funded by NHLBI (U01 HL66579)