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Fibronectin is transcriptionally upregulated during murine colitis: Potential role of fibronectin in epithelial cell attachment
Author(s) -
Kolachala Vasantha L,
Bajaj Rahul,
Wang Lixin,
R Vinu chandran,
Ruble Brittani K,
Jd Ritzenthaler,
J Roman,
Merlin Didier,
Sitaraman Shanthi V
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a588-d
Subject(s) - fibronectin , downregulation and upregulation , colitis , cell , pathogenesis , inflammation , immunohistochemistry , microbiology and biotechnology , chemistry , cell migration , cancer research , biology , immunology , gene , biochemistry
The expression of FN during intestinal inflammation is not known. The purpose of this study was to evaluate fibronectin expression and its potential role in colitis. FVB/NJ mice and transgenic mice expressing FN‐promoter were given water or 3% dextan sodium sulfate (DSS) for 5 days. Colitis was determined by clinical and histological scores. Electrical Cell Impedance Sensing was used for cell attachment and wound healing studies. FN protein level in colitic tissue was upregulated by 2.4 fold compared to normal colon. FN promoter assays revealed two‐fold increase in DSS‐treated FN promoter transgenic mice. Immunohistochemistry revealed predominant FN expression in epithelial cells. Pretreatment of Caco2 –BBE cells with FN upregulated a5 integrin, activation and nuclear translocation of NF‐kb‐p65 in a dose and time dependent manner. Further, FN enhanced Caco2‐BBE cell attachment and FN‐mediated NF‐kB activation was required for its effect on cell attachment. Collectively, these findings demonstrate an important role of FN in epithelial cell attachment and have mechanistic implications in understanding the role of FN in the pathogenesis of colitis.