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Desmin and αB‐crystallin are increased with chronic run training
Author(s) -
Gibson John,
Nelson Daniel,
Woolstenhulme Mandy,
Parcell Allen C
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a579-c
Subject(s) - desmin , myofibril , endurance training , crossbridge , actin , myosin , chemistry , medicine , biology , endocrinology , microbiology and biotechnology , immunohistochemistry , vimentin
We have investigated the concentrations of the intermediate filament protein desmin and the small heat shock protein αB‐crystallin in human skeletal muscle. Desmin integrates myofibrils at the level of the Z‐line to facilitate force transmission. αB‐crystallin is purported to play a role in desmin filament assembly and stabilization. We have previously demonstrated increases in desmin content in response to short‐term progressive resistance exercise and high‐intensity sprint cycle training. To assess the impact of chronic exercise training subjects were selected from a population of highly‐trained, competitive endurance athletes and normally active, untrained individuals. Muscle biopsies were obtained from the mid‐portion of the vastus lateralis and equal amounts of total protein homogentates were analyzed with immunoblotting techniques. Endurance athletes possessed greater (P<0.05) amounts desmin (28±3%) and αB‐crystallin (37±3%) compared to their untrained counterparts. These data demonstrate that long‐term endurance exercise with an eccentric component elevates important structural proteins in muscle possibly increasing the muscles capacity to withstand eccentric loading and transmit forces generated from actin‐myosin crossbridge formation.