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The influence of physical training on the angiopoietin and VEGF‐A systems in human skeletal muscle
Author(s) -
Gustafsson Thomas,
Rundqvist Helene,
Norrbom Jessica,
Rullman Eric,
Jansson Eva,
Sundberg Carl Johan
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a576-a
Subject(s) - basal (medicine) , messenger rna , endocrinology , medicine , angiogenesis , vegf receptors , skeletal muscle , protein expression , biology , gene , diabetes mellitus , biochemistry
11 subjects performed one‐legged exercise program for 5 weeks. The subjects exercised one leg for 45 min with restricted blood flow, followed by exercise with the other leg at the same absolute workload with unrestricted blood flow. Gene expression was measured in m vastus lateralis before the training period, after 10 days and after five weeks of training as well as 2 h after the first and last exercise bouts. Ang‐2:Ang‐1 ratio increased to a greater extent in the R‐leg. The changes in Ang‐2 mRNA were followed by changes at the protein level. In the R‐leg, VEGF‐A mRNA expression responded transiently after acute exercise before and after the training program. There was a concurrent increase in basal VEGF‐A protein and VEGFR‐2 mRNA in the R‐leg.. Ki‐67 mRNA showed a greater increase in the R‐leg and the protein was localized to the endothelial cells. Thus, the increased VEGF‐A protein level is suggested to be due to short mRNA burst induced by each exercise bout. The concurrent increase in the Ang‐2:Ang‐1 ratio and the VEGF‐expression combined with the higher level of Ki‐67 mRNA in the R‐leg indicate that coordinated changes in these systems are of importance in exercise‐induced angiogenesis.