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A single bout of exercise activates matrix metalloproteinase in human skeletal muscle
Author(s) -
Rullman Eric,
Rundqvist Helene,
Wågsäter Dick,
Fischer Helene,
Eriksson Per,
Sundberg CarlJohan,
Jansson Eva,
Gustafsson Thomas
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a575-c
Subject(s) - endostatin , skeletal muscle , medicine , endocrinology , matrix metalloproteinase , metalloproteinase , messenger rna , chemistry , vegf receptors , gene , biochemistry
Aims: to characterize changes in matrix metalloproteinase (MMP), endostatin and VEGF‐A expression in skeletal muscle in response to a single bout of exercise in humans; to determine if any exchange of endostatin and VEGF‐A between circulation and exercising leg occur with exercise. Ten healthy males performed 65 min of cycle exercise, muscle biopsies were obtained from the vastus lateralis muscle at rest, immediately and 120 min after exercise. Measurements of mRNA of MMP‐2, MMP‐9, MMP‐14, TIMP‐1 and VEGF; protein levels of VEGF and endostatin; and activity of MMP were performed. Femoral arterial‐venous differences of VEGF‐A and endostatin were determined. MMP‐9 mRNA and activity increased in the skeletal muscle. No increase of endostatin was observed in the skeletal muscle or plasma following exercise. An increase in skeletal muscle VEGF‐A mRNA and protein were induced by exercise, but without any uptake from the circulation. However, plasma VEGF‐A concentration decreased following exercise. Thus, a single bout of exercise activated the MMP‐system without any change in tissue endostatin protein level; increased VEGF‐A protein levels due to increased skeletal muscle gene expression.