Premium
Acute treadmill‐running increases intracellular signaling kinases that phosphorylate eNOS in the vasculature of mice
Author(s) -
McMillin S,
Filho J de Amorim,
Abel ED,
Symons JD
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a573-a
Subject(s) - protein kinase b , enos , ampk , phosphorylation , kinase , medicine , creb , protein kinase a , endocrinology , chemistry , activator (genetics) , nitric oxide synthase , nitric oxide , biochemistry , transcription factor , receptor , gene
Results from cultured endothelial cells and isolated blood vessels indicate that mechanical forces and increased perfusion rate, respectively, phosphorylate (p) intracellular signaling kinases [e.g., Akt/protein kinase B (Akt), protein kinase A (PKA), adenosine monophosphate‐activated protein kinase (AMPK)], and endothelial nitric oxide synthase (eNOS). Functional hyperemia occurs in metabolically active tissues during dynamic exercise. Hyperemia increases mechanical forces along the endothelial surface of the arterial wall. We hypothesized that acute exercise activates intracellular signaling kinases and eNOS in the vasculature. Mice completed 46±5 min of treadmill‐running (20 m/min @ 5% grade; EX; n=9) or were exposed to treadmill noise/vibration (SED, n=8). Immediately thereafter, iliac and femoral arteries were isolated and prepared to assess: p‐Akt at serine (S) 473 and threonine (T) 308; p‐cAMP response element binding protein at S133 (CREB; a downstream target of PKA), p‐AMPK T172, and p‐eNOS S1177. Results are presented as p‐protein : total (t) protein (mean±SE). These findings indicate that acute exercise increases (p<0.05) vascular p‐Akt S473 and p‐CREB S133, but not p‐Akt T308 or p‐AMPK T172. Because maximal Akt activity requires both S473 and T308 phosphorylation, PKA might be the important activator of p‐eNOS S1177 in the vasculature in response to exercise. AHA GIA 0655222Y.