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Protein concentration of pleural liquid, peritoneal liquid and plasma measured in mice
Author(s) -
LaiFook Stephen J.,
Houtz Pamela K.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a554-d
Subject(s) - peritoneal fluid , centrifugation , medicine , chemistry , urology , chromatography
Previous results in rats showed that (total) protein concentration (C) was slightly greater in peritoneal liquid than in pleural liquid and this behavior together with a relatively high pleural‐to‐plasma C ratio pointed to an unrestrictive transport across the diaphragm ( J. Appl. Physiol ., 99 : , 2005). Balb/c mice (21 g wt, n = 6) were anesthetized and euthanatized. By dissection into the peritoneal and chest cavities, 1 μl samples of peritoneal and pleural liquid were collected using a Finnpipette. Blood (0.5 ml) was withdrawn from the jugular. Plasma was separated from the blood by centrifugation. Protein concentration of 1 μl samples was measured by a protein assay (Coomassie Plus). Where possible, measurements were made in duplicate or triplicate and averaged. Pleural liquid C was limited to 1–3 samples from each animal. Pleural, peritoneal, and plasma C averaged 2.0 ± 0.31 (SD), 2.0 ± 0.37 and 4.5 ± 0.24 g/dl, respectively. Pleural‐to‐plasma C ratio averaged 0.44 ± 0.07 while pleural‐to‐peritoneal C ratio averaged 0.99 ± 0.11. Results are consistent with pleural liquid and protein derived predominantly by transport of peritoneal liquid into the pleural space across an unrestrictive diaphragm. Supported by NIH grants HL 36597 and HL 40362.