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Daily genistein injections stimulate increased anion secretion across murine intestine.
Author(s) -
AlNakkash Layla,
Batia Lyn,
Bhakta Minoti
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a542-a
Subject(s) - genistein , forskolin , endocrinology , medicine , bumetanide , chemistry , secretion , basal (medicine) , ussing chamber , ion transporter , biochemistry , stimulation , membrane , insulin
To determine effects of daily genistein injections on intestinal chloride (Cl) secretion, female mice (C57BL/6J, age 2 month) were injected with either: 600 mg/kg genistein/day (600G) or genistein‐free (0G, DMSO vehicle control). After 2 weeks of injections, jejuna were isolated and intestinal Cl secretion measured by Ussing chamber electrophysiology (short circuit current, Isc, μA/cm 2 ). Basal Isc was significantly increased (P<0.05) in mice injected with 600G (126.2±16.5, n=9) compared to those injected with 0G (43.5±14.2, n=8). Forskolin (10μM bilateral) increased Isc in both injection groups. Steady‐state forskolin‐stimulated Isc was significantly increased in mice injected with 600G (188.4±21.0, n=9) compared to those injected with 0G (101.0±28.1, n=8). The contribution of Cl to this increased intestinal anion secretion was further examined; first, the bumetanide‐sensitive Isc was significantly greater (P<0.05) in the 600G injected mice (−23.8±4.6, n=9) compared to those injected with 0G (−9.8±3.4, n=8) and second, Cl‐free ringer reduced basal Isc by 85% in mice injected with 600G (n=3). Effects of reducing both daily dose of genistein and duration of injection study will be examined. These data provide a means to ameliorate transepithelial intestinal Cl secretion utilizing daily injections of the naturally occurring phytoestrogen, genistein.