Premium
Vascular ageing related remodeling and differential expression analysis of associated genes
Author(s) -
Bai Xiaojuan,
Liu Qiang,
Li Hong,
Chen Xiangmei
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a529
Subject(s) - ageing , gene expression , gene , vascular remodelling in the embryo , biology , microbiology and biotechnology , genetics , endocrinology
Objective : To observe the morphological changes in vascular ageing–related remodeling in healthy rats during natural aging, screen out genes related to remodeling, analyze the relation between gene differential expression and morphological changes in remodeling, and illustrate possible gene regulation mechanism of vascular ageing–related remodeling. Method: On the base of regular observation of aortic macrostructure and microstructure changes, Gene Fishing differential display, RT‐PCR and Western blot was used to analyze and confirm genes related to the ageing remodeling and their differential expression dynamically in the 4, 10, 16 and 22 months old rats respectively. Results : With a slow startup, a obvious speedup and a following speed‐down, the vascular ageing–related remodeling is not a constant velocity process. The phasic expression of Cacna1c, p22‐phox, SDF‐1α was consistent with morphological changes closely in vascular ageing–related remodeling. Conclusion: The phasic expression of Cacna1c, p22‐phox, SDF‐1α may be the inherent gene regulation mechanism for vascular ageing–related remodeling. Cacna1c, p22‐phox, SDF‐1α seem to be the key genes for startup, speedup and keeping up of vascular ageing–related remodeling and Cacna1c, p22‐phox, SDF‐1α will be the promising molecular target for anti‐vascular aging.