Premium
Nitrc oxide breathing prevents vasoconstriction after tetrameric hemoglobin infusion
Author(s) -
Yu Binglan,
Raher Michael J,
Ichinose Fumito,
Liu Rong,
Bloch Kenneth D,
Zapol Warren M
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a525-b
Subject(s) - vasoconstriction , hypoxic pulmonary vasoconstriction , hemoglobin , methemoglobin , hemodynamics , anesthesia , nitric oxide , medicine , chemistry , pharmacology
Objective: The objective of our study was to investigate if pretreatment with inhaled nitric oxide (iNO) could prevent the vasoconstriction and subsequent systemic hypertension caused by infusion of an autologous tetrameric hemoglobin (Hb) solution in mice. Methods: Following a period of breathing either air or NO gas (80 ppm), tetrameric Hb solution (4 g/dl) prepared from whole blood of C57BL/6 mice was administered IV (0.012 ml/g BW). Systolic arterial pressure (SAP) was measured in awake mice by the tail‐cuff, and in anesthetized mice by invasive hemodynamics. Results: IV infusion of tetrameric Hb solution increased SAP from 118±3 to 143±7 mmHg (p<0.05) at 15 min in mice breathing air alone. In contrast, mice pretreated with iNO (80 ppm, 15 min), SAP was not changed after IV administration of tetrameric Hb while breathing air (118±3 vs. 120±2 mmHg, p=NS). These findings were confirmed using invasive hemodynamic methods. Breathing NO prior to Hb administration did not increase the metHb level in plasma (2.1±0.8%, 10 min after administration). However, 80 ppm NO breathing, continued for 10 min after tetrameric Hb administration, markedly increased plasma metHb level (59.8±0.6%). Conclusions: Pretreatment of mice by breathing 80 ppm NO for 15 min prevents the systemic vasoconstrictor effects of IV tetrameric Hb infusion without causing methemoglobinemia. This study was supported by a grant from the USPHS (HL 42397).