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Influence of physical activity and the T‐786C eNOS polymorphism on nitric oxide concentrations and superoxide dismutase activity
Author(s) -
Zago Anderson Saranz,
Fenty Nicola,
Park JungJun,
Park Joon,
Ferrell Robert,
Ellis Tina,
Attipoe Selasi,
Brown Michael
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a524
Subject(s) - enos , nox , superoxide dismutase , nitric oxide , chemistry , oxidative stress , nitric oxide synthase , medicine , endocrinology , biochemistry , organic chemistry , combustion
Endothelial nitric oxide synthase (eNOS) and superoxide dismutase (SOD) both contribute to the levels of bioactive nitric oxide (NO) and can be stimulated by shear stress. Therefore, this study investigated the influence of aerobic exercise training (AEX) and the T‐786C eNOS gene polymorphism on circulating levels of NO metabolites (NOx) and SOD activity. Twenty‐seven sedentary, 60±6.8 years‐old, pre‐hypertensive subjects participated in this study. Blood samples were collected after 12 hr overnight fast for genotyping, NOx and SOD assays. Genomic DNA was isolated from peripheral mononuclear cells and genotyping was done by standard PCR methods. The NOx and SOD assays were done before and after 6 mo of AEX. Baseline NOx levels were significantly lower in the TC+CC group compared to the TT group (15.2±3.1 vs. 18.4±4.4 μM, p<0.03). However, after 6 mo of AEX, NOx levels increased in the TC+CC group but did not change in the TT group (16.9±3.1 vs. 18.1±3.7 μM, p<0.03). There were no significant differences in SOD activity in either genotype group before or after AEX (TT: 1.06±0.8 vs 1.17±1.0 U/ml; TC+CC: 0.74±0.3 vs 0.89±0.3 U/ml). These results suggest that lower NOx levels at baseline in the TC+CC group occur because of decreased eNOS expression instead of NO scavenging mechanisms. The absence of changes in SOD activity may suggest that antioxidant capacity is not impaired in these pre‐hypertensive subjects. FAPESP – 04/07779‐4 (AS. Zago), KO1AG019640 (MD. Brown), AG15384, AG17474, and AG00268 (JM. Hagberg).