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TRPV1 and Fos staining in the forebrain of rats following water deprivation
Author(s) -
Carlisle Jennifer N,
Martinez Michelle M,
Little Joel T,
Carreno Flavia R,
Ji Lisa L,
Matthews Kristen I,
Cunningham J Thomas
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a516-b
Subject(s) - supraoptic nucleus , medicine , endocrinology , lamina terminalis , hypothalamus , forebrain , chemistry , median preoptic nucleus , trpv1 , vasopressin , plasma osmolality , c fos , nucleus , angiotensin ii , biology , receptor , gene expression , subfornical organ , transient receptor potential channel , central nervous system , blood pressure , microbiology and biotechnology , gene , biochemistry
Previous studies indicate that a splice variant of the transient receptor potential (TRP) V1 is expressed in the supraoptic nucleus (SON) and that it might play a prominent role in the osmosensitivity of these cells. This hypothesis was tested by using immunohistochemistry for TRPV1 (Neuromics C‐terminus directed Guinea Pig anti‐TRPV1; 1:1000) and Fos (Oncogene rabbit anti‐Fos; 1:30,000) following 48 h water deprivation (WD) in adult male rats. In euhydrated control (C) rats and WD rats, TRPV1 staining was present in the SON, the orgnum vasculosum of the lamina terminalis (OVLT), the median preoptic nucleus (MnPO), and the paraventricular nucleus of the hypothalamus (PVN). Water deprivation significantly increased plasma osmolality, plasma protein, and hematocrit. In the SON, water deprivation significantly increased the number of Fos positive cells (C (n = 8) 4.6 ± 1.8, WD (n = 7) 60.0 ± 11.3; P < 0.05) and most of the cells were also positive for TRPV1 (C (n = 8) 4.0 ± 1.6, WD (n = 7) 50.6 ± 7.2; P < 0.05). Similar results were observed in the OVLT [Fos: (C (n = 8) 10.1 ± 1.9, WD (n = 5) 116.5 ± 25.7; P < 0.05); TRPV1 + Fos: (C (n = 8) 9.5 ± 1.7, WD (n = 5) 96.9 ± 25.2; P < 0.05)], MnPO [Fos: (C (n = 6) 2.0 ± 0.9, WD (n = 6) 41.4 ± 11.3; P < 0.05); TRPV1 + Fos: (C (n = 6) 1.5 ± 0.8, WD (n = 6) 34.0 ± 8.9; P < 0.05)] and the PVN [Fos: (C (n = 4) 3.9 ± 2.3, WD (n = 4) 38.7 ± 4.8; P < 0.05); TRPV1 + Fos: (C (n = 4) 3.1 ± 1.8, WD (n = 4) 35.1 ± 3.6; P < 0.05)]. These results are consistent with the hypothesis that TRPV1 participates in osmosensing in the hypothalamus and circumventricular organs. ( HL062579 , DK57822)