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B type Natriuretic Peptide (BNP) Decreases Gastric Emptying in Mice
Author(s) -
Addisu Anteneh,
Gower William R.,
Landon Carol S.,
Dietz John R.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a511
Subject(s) - gastric emptying , medicine , natriuretic peptide , endocrinology , preload , stomach , heart failure , gastroenterology , hemodynamics
BNP is released from ventricular myocytes and is elevated in states such as acute myocardial infarction and heart failure. Such states are in turn associated with symptoms of impaired gastric emptying in humans. Our prior studies have shown that BNP and other natriuretic peptides decrease intragastric pressure. The purpose of this study was to determine if BNP affects gastric emptying. Conscious C57BL/6 (WT) & Natriuretic Peptide Receptor A knock out (KO) mice were given 10ng/g of BNP i.v. in 100 μl of modified Krebs or vehicle. The mice were then gavaged with 70KD FITC‐dextran and sacrificed 30 minutes later. The stomach was separated and the intestine divided into 8 equal segments, each flushed with 3 ml of buffer and centrifuged for 10 minutes. Fluorescence of the supernatant fluid was measured. The percent gastric emptying rate was compared in BNP treated vs. control for both WT and KO mice. Gastric emptying for WT mice was 87.8 ± 0.02% for BNP treated vs. 97.3 ± 0.03 % for control (P<0.05). For KO mice emptying was 96 ± 0.01% vs. 91.1± 0.03% (P >0.05). Thus, the present results indicate that BNP decreases gastric emptying. We postulate that BNP could be responsible for some of the gastrointestinal symptoms seen in heart failure and this effect may be aimed at decreasing preload through decreased water and electrolyte absorption from the GI tract. (Support: V. A. Merit Award to WR Gower Jr. and NSF IGERT grant 0221681)