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Dynamic studies using flowing blood in a controlled system
Author(s) -
Filho Ivo Torres,
Filler Robert,
Ellis Robert,
Berger Brian,
Terner James,
Torres Luciana,
Pittman Roland,
Ward Kevin
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a492-a
Subject(s) - oxygenator , hemoglobin , chemistry , raman spectroscopy , analytical chemistry (journal) , oxygenation , blood flow , biomedical engineering , nuclear magnetic resonance , materials science , optics , chromatography , anesthesia , medicine , biochemistry , cardiopulmonary bypass , physics
A system was developed for in vitro studies using flowing blood. The system (priming volume: 5–10 ml) includes a computer‐controlled pump, gas mixer, oxygenator and interconnected tubing that allows the flowing blood to be exposed to various O 2 and CO 2 tensions, yielding controlled levels of hemoglobin (Hb) O 2 saturation (SO 2 ). Therefore, blood sampled at different O 2 partial pressures (PO 2 ) can be used to obtain Hb O 2 dissociation curves and to determine the Hb O 2 affinity. SO 2 and PO 2 can also be measured optically using Raman micro‐spectroscopy and phosphorescence quenching, respectively. In SO 2 determinations, scattered Raman light from laser‐excited regions is directed to a spectrometer connected to a cooled CCD detector. The intensities of up to 6 Raman peaks can be used, corresponding to oxidation state marker bands of Hb, designated as ν4, ν10 and ν19. Since the blood flow and the pressure drop are measured, alterations in blood viscosity are continuously recorded. Changes in blood cell morphology and interactions between blood cells and endothelial cells can be evaluated at each oxygenation level using video‐microscopy. The system allows critical physiological variables to be studied using blood sampled from animals and patients. If the medical history of a patient is known, it is possible to correlate the clinical findings with the information obtained using this system and his/her own blood. Support: ONR and NIH