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EARLY ACTIVATION OF NUCLEAR FACTOR KAPPA B AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN ACUTE LUNG INJURY
Author(s) -
Esechie Aimalohi,
Enkhbaatar Perenlei,
Jonkam Collette,
Traber Lillian D,
Traber Daniel L
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a491-b
Subject(s) - lung , medicine , vascular endothelial growth factor , smoke inhalation , cytokine , total body surface area , inhalation , anesthesia , vegf receptors
Burn and smoke inhalation‐induced acute lung injury is often associated with production of reactive oxygen species leading to a huge host inflammatory response. The objective was to mechanistically determine the onset of NF?B activation and translocation and the production of VEGF‐A cytokine in response to oxidant production in a clinically relevant ovine model of acute lung injury. METHODS: Female sheep (N=18) were operatively prepared for study. Seven days after recovery, tracheostomy was performed under deep anesthesia. Sheep (N=3) were randomly allocated and sacrificed at the following time points (hrs): Sham (N=3, non‐injured, non‐treated); T=4, 8, 12, 24 (3rd degree 40% total body surface area flame burn and insufflation with 48 breaths of cotton smoke). Protein expression was determined using immunoblotting technique and plasma volume was measured using and indicator dilution technique with indocyanine green dye. Data is reported as mean ± SE. RESULTS: VEGF‐A protein expression peaked T=8 post BS; plasma volume differed at baseline (1645.8±396.5), T=8 (1137±164 ml) and T=18 (1442.9±50.8). I?B?[pSpS32/36] expression in cytosol was significant at T=12 corresponding to early p65 nuclear expression at T=4, T=8. CONCLUSION: Early intervention to attenuate oxidant production after acute lung injury may be beneficial in managing microvascular dysregulation. Supported by PO12GM066312; R01GM60688; SBI 8450; SBI 8954; SBI 8820