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Expression of gicerin by NGF
Author(s) -
Taira Eiichi,
Kohama Keiko
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a487-a
Subject(s) - microbiology and biotechnology , mapk/erk pathway , luciferase , biology , western blot , promoter , gene , messenger rna , northern blot , gene expression , extracellular , signal transduction , transfection , genetics
Gicerin is a cell adhesion that consists of five immunoglobulin‐like loop structures in an extracellular domain, a single transmembrane region, and a short cytoplasmic tail. Gicerin participates in nuerite extension and structural organization of nervous system. Expression of gicerin is highly observed during development and dramatically decreased after birth. To elucidate the mechanisms of regulation of gicerin expression, we cloned a mouse gicerin gene. The gene has a TATA less promoter with several Sp1 and a CRE binding sites. On the other hand, we have found that the expression of gicerin in PC12 cells was regulated by NGF. Northern blot analysis and a quantitative PCR revealed that NGF induced two folds increase of gicerin mRNA. To characterize its promoter region we examined the promoter activity in PC12 cells by luciferase assay. It showed that NGF‐induced transcriptional activity of the gicerin promoter was mediated by the CRE site. PD98059, U0126 and SB203580 significantly attenuated the increase of the promoter activity, suggesting the involvement of MAPK, ERK and p38 pathway.