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Proteomic profiling of salt‐induced changes in protein expression in PPARα null mice
Author(s) -
Obih Patience,
Oyekan Adebayo
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a477
Subject(s) - peroxisome proliferator activated receptor , downregulation and upregulation , chemistry , fold change , peroxisome , kidney , gene expression , transcription factor , proteome , biochemistry , biology , gene , microbiology and biotechnology , medicine , endocrinology
PPARs are ligand‐activated nuclear transcription factors that regulate βoxidation of fatty acids in various tissues including the kidney. PPARα is a putative target in the regulation of cardiovascular function. We have previously demonstrated that PPARα regulates renal Na + transport and contributes to salt‐sensitive hypertension. However, the proteins involved in PPARα‐mediation of salt handling and their differential expression are unknown. In this study, we used the 2D‐DIGE – LC‐MS/MS technology to profile and identify proteins that are differentially expressed in tissues from PPARα KO and WT mice as a function of normal (0.3% NaCl, NS) and high (8% NaCl, HS) salt diets. Initial biological variation analysis using DeCyder software (v. 6.0) revealed the presence of 20 proteins that are upregulated and 9 proteins that are downregulated in the kidney (K), aorta (A), and heart (H) tissues from PPARα KO and WT mice. A multimodality comparison of the 29 differentially expressed proteins showing ≥1.5‐fold change, ≥20% appearance at p≤0.05 in K, A, and H revealed 11 proteins (WT; NS vs HS), 9 proteins (NS; WT vs KO), 3 proteins (HS; WT vs KO), and 6 proteins (KO; NS vs HS). Some of the proteins identified by LC‐MS/MS are involved in fatty acid oxidation (FAO). These results demonstrate that: salt elicits a differential protein expression in PPARα KO and WT mice and the differentially expressed proteins involved in regulation of FAO pathway contribute to regulation of renal function and blood pressure.