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Anterograde Tracing of A1 and A5 Efferents Using Phenotypically Restricted Lentivirus Vector Mediated Reporter Gene Expression
Author(s) -
Card J. Patrick,
Sved Judith C.,
Corbin Tshona,
Shively Charise,
Li Xia,
Yen JenShew,
Raizada Mohan K.,
Shan Zhiying,
Sved Alan F.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a474-a
Subject(s) - efferent , neuroscience , biology , catecholamine , efferent neuron , spinal cord , retrograde tracing , dopamine , microbiology and biotechnology , nucleus , afferent
Projections of brain stem catecholamine cell groups are difficult to document since their projection fields overlap. Recently we developed a novel approach for anterograde tracing of catecholamine neurons and used it to map the efferent projections of C1 RVLM neurons. This was achieved using a lentivirus vector that expresses green fluorescent protein under the control of a synthetic dopamine‐β‐hydroxylase promoter. The reporter protein fills the axonal arbor of catecholamine neurons, including intervaricose segments. We used this approach to map the efferent projections of A1 and A5 catecholamine neurons. Differential projections of A1 and A5 were demonstrated. A5 gave rise to a dense spinal projection and also projected to brain stem and diencephalon. A1 preferentially gave rise to ascending projections but did not project to spinal cord. These data reiterate the functional heterogeneity of the catecholamine cell groups and provide further insight into the role of the A1 and A5 neurons in autonomic and endocrine regulation. ( RRO18604 , HL55786, HL76312, Neurogenic Cardiovascular Disease Consortium)